Journal: Radiation research

Article Title: Radiation-Induced Long Noncoding RNAs in a Mouse Model after Whole-Body Irradiation

doi: 10.1667/RR14891.1

Figure Lengend Snippet: RT2 lncRNA PCR Array mouse lncFinder profiling of several characterized lncRNAs as radiation targets. Panels A–C: Bar graphs show dose responsiveness of Pvtl, Gm14005 and Gas5 and Snhg8 at 16, 24 and 48 h postirradiation, respectively. Relative expressions (2−dCt) are plotted across doses. dCt was calculated against endogenous control gene, Gt(ROSA)26Sor. All graphs show mean ± SD from three animals/dose/time point. ANOVA followed by a Dunnett’s post hoc test (treatments compared to control) was performed to compute statistical significance. *P < 0.05; **P < 0.01 and ***P < 0.001.

Article Snippet: RT 2 lncRNA PCR Array Mouse lncFinder For lncRNA expression analysis we used RT 2 lncRNA PCR lncFinder array (product no. 330721, cat. no. LAMM-0012A-24; QIAGEN) with predispensed primers against 84 known lncRNAs in a 96-well plate format.

Techniques:

Journal: Radiation research

Article Title: Radiation-Induced Long Noncoding RNAs in a Mouse Model after Whole-Body Irradiation

doi: 10.1667/RR14891.1

Figure Lengend Snippet: Comparison of 1110038B12Rik and Tug1 between the microarray and the lncFinder data. Panels A and B: Line graphs showing downregulation of 1110038B12Rik in response to increasing radiation doses at the 16 h time point in the microarray and lncFinder data, respectively. Panels C and D: Line graphs showing upregulation of Tug1 in response to increasing radiation doses at the 48 h time point in the microarray and lncFinder data. *P < 0.05; **P < 0.01 and ***P < 0.001.

Article Snippet: RT 2 lncRNA PCR Array Mouse lncFinder For lncRNA expression analysis we used RT 2 lncRNA PCR lncFinder array (product no. 330721, cat. no. LAMM-0012A-24; QIAGEN) with predispensed primers against 84 known lncRNAs in a 96-well plate format.

Techniques: Microarray

Journal: Radiation research

Article Title: Radiation-Induced Long Noncoding RNAs in a Mouse Model after Whole-Body Irradiation

doi: 10.1667/RR14891.1

Figure Lengend Snippet: Status of p53 LncRNA targets and Gm14005 in mouse embryonic fibroblast cells. Panel A: qRT-PCR analysis for Cdkn1a, Trp53cor1 and Dino in MEFs. Panel B: qRT-PCR analysis for Gm14005 in MEFs. Each data point depicts mean ± SD relative expression from three independent experiments across different doses compared to 0 Gy. Gapdh was used as the endogenous normalizing control to calculate fold changes. Statistical significance was calculated using unpaired t test. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

Article Snippet: RT 2 lncRNA PCR Array Mouse lncFinder For lncRNA expression analysis we used RT 2 lncRNA PCR lncFinder array (product no. 330721, cat. no. LAMM-0012A-24; QIAGEN) with predispensed primers against 84 known lncRNAs in a 96-well plate format.

Techniques: Quantitative RT-PCR, Expressing

Journal: Frontiers in Physiology

Article Title: Microarray Analysis of lncRNA and mRNA Reveals Enhanced Lipolysis Along With Metabolic Remodeling in Mice Infected With Larval Echinococcus granulosus

doi: 10.3389/fphys.2020.01078

Figure Lengend Snippet: The identification and validation of differentially expressed mRNAs and lncRNAs in adipose tissues of EgPSC infected mice. The volcano plots show the distributions of (A) mRNAs and (B) lncRNAs. The x-axis indicates the fold change. The horizontal green line represents the filter criterion (threshold P ≤ 0.05); red dots, upregulated; blue dots, downregulated. (C) qRT-PCR validation of randomly selected differentially expressed mRNAs and lncRNAs from the microarray data.

Article Snippet: An Agilent Mouse lncRNA Microarray (design ID: 085631) was used in this experiment.

Techniques: Infection, Quantitative RT-PCR, Microarray